What is in the fish? Collaborative trial in suspect and non-target screening of organic micropollutants using LC- and GC-HRMS - EHESP - École des hautes études en santé publique Access content directly
Journal Articles Environment International Year : 2023

What is in the fish? Collaborative trial in suspect and non-target screening of organic micropollutants using LC- and GC-HRMS

1 SLU - Swedish University of Agricultural Sciences = Sveriges lantbruksuniversitet
2 Irset - Institut de recherche en santé, environnement et travail
3 NKUA - National and Kapodistrian University of Athens
4 Stockholm University
5 Umeå University
6 Eawag, Swiss Federal Institute of Aquatic Science and Technology, Überlandstrasse 133, Dübendorf, 8600, Switzerland
7 UFZ - Helmholtz Zentrum für Umweltforschung = Helmholtz Centre for Environmental Research
8 IBP - Institute of Biogeochemistry and Pollutant Dynamics [ETH Zürich]
9 UR - Université de Rennes
10 LERES - Laboratoire d'étude et de recherche en environnement et santé
11 EHESP - École des Hautes Études en Santé Publique [EHESP]
12 DEESSE - Département des sciences en santé environnementale
13 Bavarian Environment Agency (LfU)
14 UA - University of Antwerp
15 IPREM - Institut des sciences analytiques et de physico-chimie pour l'environnement et les materiaux
16 IFREMER - Institut Français de Recherche pour l'Exploitation de la Mer
17 BAM - Federal Institute for Materials Research and Testing - Bundesanstalt für Materialforschung und -prüfung
18 Aarhus University [Aarhus]
19 CNR - National Research Council of Italy | Consiglio Nazionale delle Ricerche
20 Istituto di Ricerche Farmacologiche "Mario Negri"
21 UPV / EHU - University of the Basque Country = Euskal Herriko Unibertsitatea
22 Catalan Institute for Water Research (ICRA)
23 UdG - Universitat de Girona
24 INSTITUTE OF ENVIRONMENTAL ASSESSMENT AND WATER RESEARCH SPANISH COUNCIL FOR SCIENTIFIC RESEARCH BARCELONA ESP
25 LEESA - Laboratoire d'Ecophysiologie et Ecotoxicologie des Systèmes Aquatiques
26 BFG FEDERAL INSTITUTE OF HYDROLOGY KOBLENZ DEU
27 Métabolomique (Plate-forme) – Metabolomics core facility
28 VITO - Flemish Institute for Technological Research
29 Fraunhofer IME - Fraunhofer Institute for Molecular Biology and Applied Ecology
Peter Haglund
  • Function : Author
André Macherius
Giulia Poma
Martin Hansen
Katrin Vorkamp

Abstract

A collaborative trial involving 16 participants from nine European countries was conducted within the NORMAN network in efforts to harmonise suspect and non-target screening of environmental contaminants in whole fish samples of bream (Abramis brama). Participants were provided with freeze-dried, homogenised fish samples from a contaminated and a reference site, extracts (spiked and non-spiked) and reference sample preparation protocols for liquid chromatography (LC) and gas chromatography (GC) coupled to high esolution mass spectrometry (HRMS). Participants extracted fish samples using their in-house sample preparation method and/or the protocol provided. Participants correctly identified 9-69% of spiked compounds using LC-HRMS and 20-60% of spiked compounds using GC-HRMS. From the contaminated site, suspect screening with participants’ own suspect lists led to putative identification of on average ~145 and ~20 unique features per participant using LC-HRMS and GC-HRMS, respectively, while non-target screening entified on average ~42 and ~56 unique features per participant using LC-HRMS and GC-HRMS, respectively. Within the same sub-group of sample preparation method, only a few features were identified by at least two participants in suspect screening (16 features using LC-HRMS, 0 features using GC-HRMS) and non-target screening (0 features using LC-HRMS, 2 features using GC-HRMS). The compounds identified had log octanol/water partition coefficient (KOW) values ranging from -9.9 to 16 and mass-to-char ge ratio (m/z) of 68 to 761 (LC-HRMS and GC-HRMS). A significant linear trend was found between log KOW and m/z for the GC-HRMS data. Overall, these findings indicate that differences in screening results are mainly due to the data analysis workflows used by different participants. Further work is needed to harmonise the results obtained when applying suspect and non-target screening approaches to environmental biota samples
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hal-04259807 , version 1 (26-10-2023)

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Wiebke Dürig, Sofia Lindblad, Oksana Golovko, Georgios Gkotsis, Reza Aalizadeh, et al.. What is in the fish? Collaborative trial in suspect and non-target screening of organic micropollutants using LC- and GC-HRMS. Environment International, 2023, 181, pp.108288. ⟨10.1016/j.envint.2023.108288⟩. ⟨hal-04259807⟩
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