Analytical techniques in metabolomics [Chapter 2] - EHESP - École des hautes études en santé publique Access content directly
Book Sections Year : 2020

Analytical techniques in metabolomics [Chapter 2]


Recent advances in metabolomics-based approaches using liquid or gas chromatography coupled to high-resolution mass spectrometry (HRMS) offer exciting perspectives to detect and identify simultaneously biomarkers of effect (i.e., disruption of endogenous metabolite profiles) and exposure (relevant xenobiotic mixtures and/or emerging compounds) accumulating in environmental and/or biological samples. Great efforts are currently being made to improve the steps of detection and identification as well as the treatment of data generated to obtain biological information. However, other steps in the general workflow on metabolomics analysis have not received the same attention. These steps fall within the concept of sample preparation and chromatographic separation, which continues to be the bottleneck in the development of analytical methods. This chapter will discuss modern separation and identification methods, with a major focus on HRMS and complementary separation techniques together with the current state of the art for sample preparation for metabolomics.
No file

Dates and versions

hal-04085253 , version 1 (28-04-2023)



Arthur David, Pawel Rostkowski. Analytical techniques in metabolomics [Chapter 2]. Environmental Metabolomics: Applications in field and laboratory studies to understand from exposome to metabolome, Elsevier, pp.35-64, 2020, 978-0128181966. ⟨10.1016/B978-0-12-818196-6.00002-9⟩. ⟨hal-04085253⟩
19 View
0 Download



Gmail Facebook X LinkedIn More